Bacteriological Profile of Acute Pyogenic Meningitis in Pediatric Age Group from a Tertiary Care Hospital of East Delhi

Prakash K Mishra; Narendra P Singh; Prerna Batra; Iqbal R Kaur

Bacteriological Profile of Acute Pyogenic Meningitis in Pediatric Age Group from a Tertiary Care Hospital of East Delhi

Prakash K Mishra, Narendra P Singh, Prerna Batra, Iqbal R Kaur

Abstract

Background and Objectives Laboratory surveillance of causative microorganism is
crucial to formulate rational empirical treatment and preventive strategies for potentially
fatal acute pyogenic meningitis (APM). This study was aimed to identify the causative
microorganism of APM in children admitted in a tertiary care hospital of east Delhi.
Materials and Methods Cerebrospinal fluid (CSF) was collected from 300 suspected
cases of APM and subjected to cytology, gram staining, biochemical analysis and culture.
Every fifth CSF sample was also subjected to antigen detection by latex agglutination
test (LAT) for Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis,
Escherichia coli, group B Streptococcus and TaqMan probe-based real time polymerase
chain reaction (RTPCR) for S. pneumoniae, H. influenzae and N. meningitidis.
Simultaneously, blood culture was also done from all these 300 cases.
Result From CSF, 19 microorganisms were isolated in which hospital-acquired multidrug
resistant Acinetobacter baumannii (9) was most common. S. pneumoniae, H. influenzae,
group B Streptococcus, E. coli, Staphylococcus aureus and Klebsiella were also identified
as a causative agent of APM. LAT detected microorganism in nine culture negative samples,
whereas RTPCR detected two additional microorganisms which were missed by LAT.
Conclusion This study highlights the emergence of MDR A. baumannii as a common
agent of APM in hospital setting and emphasise on judicious use of antibiotics to prevent
resistance.

Keywords

acute pyogenic meningitis, bacteriological profile, pediatric age group

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