Antinociceptive Activity of the Crude Methanolic Extract of Pseudocedrela kotschyi and Its Chloroform and n-butanol Fractions in Mice

Kabiru Abubakar; Nuhu Muhammad Danjuma; Bello Balkisu Maiha; Joseph Akpojo Anuka; Mun Fei Yam; Idris Bello; Usman Salisu Nasiba; Mohammed Asmawi. Zaini

Antinociceptive Activity of the Crude Methanolic Extract of Pseudocedrela kotschyi and Its Chloroform and n-butanol Fractions in Mice

Kabiru Abubakar, Nuhu Muhammad Danjuma, Bello Balkisu Maiha, Joseph Akpojo Anuka, Mun Fei Yam, Idris Bello, Usman Salisu Nasiba, Mohammed Asmawi. Zaini

Abstract

Pseudocedrela kotschyi has been extensively used in traditional medicine for the treatment of rheumatism, malaria, dysentery and epilepsy. The antinociceptive effects of the crude methanolic extract, n-butanol and chloroform fractions of P. kotschyi were investigated in different experimental models in mice: (1) hot plate, (2) tail flick, (3) acetic acid induced writhing and (4) formalin induced test. In the writhing test 200 mg/kg of the crude extract significantly (P < 0.05) reduced the number of writhes and produced an 88.1% inhibition. The chloroform and n-butanol fractions produced 98.59 and 92.62% inhibition of writhes respectively. In the formalin induced test 100 and 200 mg/kg of the crude methanolic extract significantly (P < 0.01) produced late phase analgesia. Similarly, 200 mg/kg of the chloroform fraction significantly (P < 0.01) reduced paw licking behaviour in mice in both early and late phases of the experiment. However, at the doses tested, no significant activity was found in the hot plate and the tail flick test. The results suggest that P. kotschyi methanol extract at 200 mg/kg dose is effective in non-steroidal anti-inflammatory drug type anti-nociception activities.

Keywords

Pseudocedrela kotschyi, antinociception, anti-inflammatory

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