Polymorphism of Vitamin D Receptor Gene -Taq1  (RS 731236) Associated With Plasma Vitamin D Group Levels Among Breast Cancer Patients in H. Adam Malik Hospital, Medan

Ika Waraztuty; Yahwardiah Siregar; Kamal Basri Siregar; Septelia Inawati Wanandi; Betty Betty

Polymorphism of Vitamin D Receptor Gene -Taq1 (RS 731236) Associated With Plasma Vitamin D Group Levels Among Breast Cancer Patients in H. Adam Malik Hospital, Medan

Ika Waraztuty, Yahwardiah Siregar, Kamal Basri Siregar, Septelia Inawati Wanandi, Betty Betty

Abstract

Aim Breast cancer was one of the most cancer occurred in women worldwide. Taq 1 polymorphism, a silent SNP, was thought to be genetic risk factor for breast cancer. This study was aimed to understand the relationship between vitamin D receptor gene–Taq 1 (RS 731236) polymorphism and plasma vitamin D level among breast cancer patients in RSU H. Adam Malik Medan. Material and Methods Blood sample was collected from 53 new breast cancer cases that had not received any chemotherapy. DNA isolation and gene amplification was done using PCR then followed with RFLP using Taq 1 restriction enzyme. The level of 25(OH)D was measured using ELISA. Result and Discussion Study results showed TT genotype was 92.5%, TC genotype was 7.5%, CC genotype was 0 % using Hardy-Winberg Equilibrium (HWE) p=0.77, mean value of vitamin D level in study subject was 28.16 ng/ml (CI 95%: 25.71-30.60). Fisher exact test analysis concluded that polymorphism of vitamin D receptor gene-Taq1 associated with plasma vitamin D group levels among breast cancer patients in H. Adam Malik Hospital, Medan (p= 0.033) but there was no difference in mean plasma vitamin D levels between genotype groups of Taq1 (p=0.141). Conclusion Vitamin D receptor gene-Taq1 polymorphism was associated with plasma vitamin D group level and but no significant differences in mean plasma vitamin D levels between genotype groups of Taq1.

Keywords

Polymorphism, VDR- Taq1, Vitamin D level, Breast Cancer.

Full Text:

References

Bray F, Ren JS, Masuyer E, & Ferlay J. Global estimate of cancer prevalence for 27 site in adult population in 2008. Int J Cancer. 2013; 32(5): 1133-1145.

Kementerian Kesehatan RI. 2015. Pusat data dan Informasi Kementerian Kesehatan RI. www.depkes.go.id

Kabel AM, Fahad HB. Breast Cancer: Insight into Risk Factor, Pathogenesis, Diagnosis and Management. Journal of Cancer Research and Treatment. 2015; 3(2): 28-33.

Carlberg, C. (2003). Current understanding of the function of the nuclear vitamin D receptor in response to its natural and synthetic ligands. In Vitamin D Analogs in Cancer Prevention and Therapy (pp. 29-42). Springer, Berlin, Heidelberg.

Norman, A. W. From vitamin D to hormone D: fundamentals of the vitamin D endocrine system essential for good health. Am J Clin Nutr. 2008; 88: 491-499.

Deeb, KK, Trump DL, Johnson CS. Vitamin D signalling pathway in cancer: Potential for Anticancer Therapeutics. Nat Rev Cancer.2007; 7: 684-700.

Fleet JC, DeSmet M, Johnson R, Li Y. Vitamin D and cancer: a review of molecular mechanism. Biochem J. 2012; 441(1): 61-76.

Iqbal MN, Khan TA. Association between vitamin D receptor (Cdx2, Fok1, Bsm1, Apa1, Bg11 and Poly A) gene polymorphism and breast cancer: a systematic review and meta-analysis. Tumor Biol. 2007; 39(10): 1-9.

Uitterlinden, A.G. et al. Genetics and biology of vitamin D receptor polymorphisms. Gene. 2014; 338 (2): 143-156.

Kotsner K, Denzer N, Muller CSL, Klein R, Tilegen W, Reichrats. The relevance of vitamin D receptor (VDR) gene polymorphism for cancer: a review of the literature. Anticancer Res. 2009;29: 3511-3536.

Bhanushali AA, Namrata L, Smita SK, Sandeep SC, Sarita SB, Bibhu RD. Frequency of kok1 and taq1 polymorphism of vitamin D receptor gene in Indian population and its association with 25-hydoxyvitamin D level. Indian J of Hum Genet. 2009; 15(3): 108-113.

Sari DK, Zaimah ZT, Sri L, Sunna VH et al. Vitamin D Receptor gene Polymorphism Among Indonesian woman in North Sumatera. Asian J Clin Nutr. 2007; 9(11): 44-50.

Atoum MF, Yasmeen Mohammad Al KH. Association between serum 25-hydroxy vitamin D concentration and Taq1 vitamin D receptor gene polymorphism among Jordania females with breast cancer. Chin Med J. 2017; 30(9): 1074-1078.

Waraztuty I, Siregar KB, Siregar Y. Vitamin D Receptor Gene Taq1 (rs731236) polymorphism in breast cancer patient at H. Adam Malik Hospital Medan and It’s relationship with histopathology grading. Biomed J Sci Tech Res. 2018; 6(1): 1-5.

Holick MF, Neil CB, Heike AB, Catherine MG et al. Evaluation, treatment and prevention of vitamin D deficiency: an endocrine society clinical practice guideline. J Clin Endocrinol Metab. 2018; 96(7): 1911-1930.

Brent J, Garne JP, Tengrup O, Manjer J. Age at diagnosis in relation to survival following breast cancer: a cohort study. World J Surg Oncol. 2015; 13:33.

Babita R, Kumar N, Karwasra, RK, Singh M, Malik JS, Kaura A. Reproductive risk factors associated with breast carcinoma in a tertiary care hospital of north India: A case-control Study. Indian J Cancer. 2014; 51(3): 251-255.

Khalis M, Carbotel B, Chajes V, Rinaldi S, Moskal A, Biessy C, et al. Menstrual and reproductive factors and risk of breast cancer: a case-control study in the Fez region, Morroco. Plos One. 2018;13(01): 1-12.

Wolin KY, Carson K, Colditz A. Obesity and cancer. Oncologist. 2010; 15(6): 556-565.

Karim SAM, Ghalib HHA, Fattah FHR, Gubar, MIM, Majeed AB. Height, weight and body mass index association with breast cancer risk in Iraqi Kurdish women. Case Study in Surgery. 2015;1(1) :1-7.

Curran JE, Vaughan T, Lea RA, Weinsten SR, Morrison NA and Griffiths LR. Association of vitamin D receptor polymorphism with sporadic breast cancer development. Int J Cancer. 1999; 83: 723-726.

El-Shorbagy HM, Mahmoud NH, Sabet S. Association of vitamin D receptor gene polymorphism with breast cancer risk in egyptian population. Tumor Biol. 2017; 1-9.

Guo B, Jing X, Hu X, Li F, Chen X. Association between vitamin D receptor gene polymorphism and breast cancer in a Chinese population. Int J Clin Exp Med. 2015; 8(5): 8020-8024.

Perna, L, Butterbach K, Haug U, Schottker B, Muller H, Arndt, V et al. Vitamin D Receptor Genotype rs731236 (Taq1) and Breast Cancer Prognosis.Cancer Epidemiol. Biomarkers Pre. 2013;22: 437-442.

Elsoud MRA, Ali HAS, Soheir RD, Basma HM. Study of relationship between vitamin D receptor gene polymorphism expression (Bsm I & Fok I), serum Level of Vitamin D and the risk breast cancer in Egyptian female; correlation with clinicopathological features of the disease. Int. J. of Basic and App. Med. Sci. 2016;6(2): 89-99.

Younus A, Faiz M, Yasmeen A. Serum Vitamin D in women with pre and postmenopausal newly diagnosed breast cancer in Pakistan. J. Food Nutr. Res. 2016;4(12): 828-833.

Refbacks

There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Journal of Pharmaceutical and Biomedical Sciences