Repair of Articular Cartilage Defect Using NaOH-treated PLGA-[ASP-PEG]n Scaffolds Loaded Adipose-derived Stem Cells and Bone Morphogenetic Protein-2

Li-Feng, Li; Pradeep Singh; Ping Ji; Bi Zhang; Song-Song Zhu

Repair of Articular Cartilage Defect Using NaOH-treated PLGA-[ASP-PEG]n Scaffolds Loaded Adipose-derived Stem Cells and Bone Morphogenetic Protein-2

Li-Feng, Li, Pradeep Singh, Ping Ji, Bi Zhang, Song-Song Zhu

Abstract

The aim of this study was to evaluate the feasibility of NaOH-treated PLGA-[ASP-PEG]n scaffolds with bone morphogenetic protein 2 (BMP-2) modified adipose derived mesenchymal stem cells (ADSCs) in repairing articular cartilage defects. To improve functions of chondrocytes including their adhesion, growth, differentiation and extracellular matrix synthesis, poly(lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol [PLGA-[ASP-PEG]n] scaffolds were modified via chemical etching techniques using 1 N NaOH for 10 min. Articular cartilage defects of 3-mm diameter and 5-mm depth were created in the patellar groove of the femoral articular cartilage of rabbit’s knee joints. The injury sites were treated with BMP-2 modified ADSCs/PLGA-[ASP-PEG]n and BMP-2 modified ADSCs/NaOH-treated PLGA-[ASP-PEG]n, or left empty. The defect area was
evaluated using gross and histological examination. Results demonstrated that NaOHtreated PLGA-[ASP-PEG]n scaffolds enhanced chondrocyte functions and present faster and more effective chondrogenesis compared to non-treated scaffolds. The present study demonstrated that NaOH-treated PLGA-[ASP-PEG]n scaffolds loaded BMP-2 modified ADSCs might be a promising treatment in repair of articular cartilage defect.

Keywords

articular cartilage, PLGA, ASP-PEG, BMP-2, ADSCs

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