Abstract
Objectives: This study was carried out to detect methicillin resistant staphylococcus aureus among tobacco users in the Port Sudan city, Sudan, using PCR technique to help reduce morbidity and mortality among tobacco users during the period from February to June, 2018.
Materials and Method: 100(one hundred) Mouth swabs were obtained randomly from tobacco users. The specimen was cultured on blood agar with novobiocin disc and mannitol salt agar for primary isolation of Pathogen. Identification of isolate was done by colonial morphology, gram stain and biochemical tests. Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance rate of staphylococcus aureus to Methicillin disc. Then mecA gene detection was done used PCR technique.
Results: A 70 (seventy) staphylococcus aureus was isolated from the mouth swab, and 14 (fourteen) MRSA was isolated out of this 70(seventy) staphylococcus aureus, 1(one) out of this were positive for mecA gene. MRSA isolate was found to be high among tobacco consumer for long period using rather than those who tobacco consuming for short period. The result revealed that the antimicrobial resistance of S. aureus for Methicillin was (20%).
Conclusion: This study concluded that there is correlation between tobacco use and duration of use and increasing susceptibility to MRSA infection
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