Abstract
Although the present study have small molecular target drug to treat melanoma patient with BRAF mutation still study faces the issue of treatment resistance. For BRAF wild type, there is no efficient targeted therapy. In this paper, the antiproliferation effect of quercetin in human melanoma BRAF wild type CHL-1 cells through (MTT), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay has been examined.The IC50 values of quercetin against CHL-1 cells for 24, 48 and 72 h were 17.41 ± 1.58,
14.62 ± 1.53 and 6.83 ± 1.21 ?M. The inverted microscopy images also showed that the CHL-1 cells were collapsed, and the cell nucleus were fragmented after 24 h treatment. From western blot assay it was found that after quercetin treatment 24 h, the expression of Bcl-2, one of pro-apoptotic protein, was increased, while Bax, one of anti-apoptosis protein was decreased. These results indicated that quercetin possessed
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References
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