Abstract
Objective Compound Danshen Tablets (CDT), also known as Fufang Danshen Pian in China, has been commonly used to prevent and treat cardiovascular diseases in the clinic in Asia-Pacific region, especially in China. This study investigated the effects and possible mechanism of CDT against isoproterenol-induced myocardial infarction (MI) in rats.
Methods Male rats were randomly assigned to six groups: control, model, isosorbide dinitrate (ISDN, 2.72 mg/kg, i.g.), low-dose CDT (0.315 g/kg, i.g.), medium-dose CDT (0.63 g/kg, i.g.), and high-dose CDT (1.26 g/kg, i.g.). After 10 consecutive days of administration, except for the control group, others were subcutaneously injected with 5 mg/kg isoproterenol for three consecutive days to establish MI models. Variables including electrocardiogram (ECG), cardiac function, infarct size, myocardium structure, and the activities of serum creatine kinase (CK), lactate dehydrogenase (LDH), cardiac troponin I (cTnI), nitric oxide (NO), endothelial nitric oxide synthase (eNOS), and brain natriuretic peptide (BNP) were determined.
Results CDT significantly decreased infarct size, BNP level in myocardial tissues, and the concentrations of serum CK, LDH and cTnI. In addition, CDT could remarkably increase the levels of serum NO, eNOS and ameliorate myocardial injury.
Conclusion CDT has endothelial protective effect against MI injury in rats by protecting cardiomyocytes, reducing myocardial infarct size, oxidative stress, the levels of serum myocardial enzymes (LDH and CK), cTnI, and BNP.
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References
Rizza S, Copetti M, Rossi C, Cianfarani M, Zucchelli M, Luzi A, et al. Metabolomics signature improves the prediction of cardiovascular events in elderly subjects. Atherosclerosis. 2014;232(2):260–264.
Pagidipati NJ, Gaziano TA. Estimating deaths from cardiovascular disease: a review of global methodologies of mortality measurement. Circulation. 2013;127(6):749–756.
White HD, Chew DP. Acute myocardial infarction. Lancet. 2008;372(9638):570–584.
Zheng CS, Xu XJ, Ye HZ, Wu GW, Xu HF, Li XH, et al. Computational pharmacological comparison of Salvia miltiorrhiza and Panax notoginseng used in the therapy of cardiovascular diseases. Exp Ther Med. 2013;6(5):1163–1168.
Luo J, Xu H, Chen KJ. Potential benefits of Chinese Herbal Medicine for elderly patients with cardiovascular diseases. J Geriatr Cardiol. 2013;10(4):305–309.
Sham TT, Chan CO, Wang YH, Yang JM, Mok DK, Chan SW. A review on the traditional Chinese medicinal herbs
and formulae with hypolipidemic effect. Biomed Res Int. 2014;2014:925302.
Ren-an Q, Juan L, Chuyuan L, Wenjuan F, Chunyan H, Xuemei Y, et al. Study of the protective mechanisms of Compound Danshen Tablet (Fufang Danshen Pian) against myocardial ischemia/ reperfusion injury via the Akt-eNOS signaling pathway in rats. J Ethnopharmacol. 2014;156:190–198.
Zhang Y, Wei L, Sun D, Cao F, Gao H, Zhao L, et al. Tanshinone IIA pretreatment protects myocardium against ischaemia/reperfusion injury through the phosphatidylinositol 3-kinase/ Akt-dependent pathway in diabetic rats. Diabetes Obes Metab. 2010;12(4):316–322.
Xu L, Deng Y, Feng L, Li D, Chen X, Ma C, et al. Cardio-protection of salvianolic acid B through inhibition of apoptosis network. PLoS One. 2011;6(9):e24036.
Yang D, Xie P, Liu Z. Ischemia/reperfusion-induced MKP-3 impairs endothelial NO formation via inactivation of ERK1/2 pathway. PloS one. 2012;7(7):e42076.
Wei YJ, Qi LW, Li P, Luo HW, Yi L, Sheng LH. Improved quality control method for Fufang Danshen preparations through simultaneous determination of phenolic acids, saponins and diterpenoid quinones by HPLC coupled with diode array and evaporative light scattering detectors. J Pharm Biomed Anal. 2007;45(5):775–784.
Sham TT, Chan CO, Wang YH, Yang JM, Mok DK, Chan SW. A review on the traditional Chinese medicinal herbs and formulae with hypolipidemic effect. Bio Med Res Int. 2014; 2014:925302.
XG, H WYS, MZ, LG, TG. Study of the effect of Compound Danshen Formula against myocardial ischemia in rats. Tianjin J Trad Chinese Med (Chin). 2003;20(1):23–25.
Rona G, Chappel Cl, Balazs T, Gaudry R. An infarct-like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. AMA Arch Pathol. 1959;67(4):443–455.
Bertinchant J, Robert E, Polge A, Marty-Double C, Fabbro-Peray P, Poirey S, et al. Comparison of the diagnostic value of cardiac troponin I and T determinations for detecting early myocardial damage and the relationship with histological findings after isoprenaline-induced cardiac injury in rats. Clin Chim Acta. 2000;298(1–2):13–28.
Xu SY, Bian RL, Chen X. Methodology of Pharmacological Experiments. People’s Medical Publishing House; Beijing: China. 2002.
Thygesen K, Alpert JS, White HD, Jaffe AS, Apple FS, Galvani M, et al. Universal definition of myocardial infarction. Circulation. 2007;116(22):2634–2653.
Sivaranjani N, Venkataraman D, Elango G, Rao SV, Ravi Kiran VS. Evaluation of cardiac specific Troponin T as a specific and sensitive biomarker over Creatine Kinase-MB in Acute Myocardial Infarction patients-A correlation analysis study. Int J Biomed Res. 2014; 5(2):121–123.
Amani M, Jeddi S, Ahmadiasl N, Usefzade N, Zaman J. Effect of HEMADO on level of CK-MB and LDH enzymes after ischemia/ reperfusion injury in isolated rat heart. BioImpacts. 2013;3(2):101–104.
Reichlin T, Hochholzer W, Bassetti S, Steuer S, Stelzig C, Hartwiger S, et al. Early diagnosis of myocardial infarction with sensitive cardiac troponin assays. N Eng J Med. 2009;361(9): 858–867.
Mayr A, Mair J, Schocke M, Klug G, Pedarnig K, Haubner BJ, et al. Predictive value of NT-pro BNP after acute myocardial infarction: relation with acute and chronic infarct size and myocardial function. Int J Cardiol. 2011;147(1):118–123.
Alderton WK, Cooper CE, Knowles RG. Nitric oxide synthases: structure, function and inhibition. Biochem J. 2001;357(3): 593–615.
Loke KE, McConnell PI, Tuzman JM, Shesely EG, Smith CJ, Stackpole CJ, et al. Endogenous endothelial nitric oxide synthase-derived nitric oxide is a physiological regulator of myocardial oxygen consumption. Circ Res. 1999;84(7):840–845.
Radomski MW, Palmer RM, Moncada S. Endogenous nitric oxide inhibits human platelet adhesion to vascular endothelium. Lancet. 1987;2(8567):1057–1058.
Ma XL, Weyrich AS, Lefer DJ, Lefer AM. Diminished basal nitric oxide release after myocardial ischemia and reperfusion promotes neutrophil adherence to coronary endothelium. Circ Res. 1993;72(2):403–412.
Ross R. Atherosclerosis—an inflammatory disease. N Eng J Med. 1999;340(2):115–126.
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