hsa-miR- 574-5p Down Regulates the Expression of CBR1 in A549 Cells and Enhances their Invasive Ability

Aijun Liu; Zhiming Liang; Jianping Zhang

hsa-miR- 574-5p Down Regulates the Expression of CBR1 in A549 Cells and Enhances their Invasive Ability

Aijun Liu, Zhiming Liang, Jianping Zhang

Abstract

Objective To explore the impact of CBR1expression on A549 cell proliferation and invasive ability, and investigate the regulatory function of hsa-miR-574-5p on these processes.

Methods A549 cells with high and low CBR1 expression were established by transfection with a CBR1-pENTER plasmid and an hsa-miR-574-5p analog, respectively, MTT assays were performed to measure cell proliferation ability, RT-PCR and western blot were used to detect the expression of CBR1, and E-cadherin, and transwell migration assays were used to detect the invasive ability of the cells.

Results Cells transfected with CBR1-pENTER showed increased CBR1 and E-cadherin expression and reduced invasive ability; those transfected with hsa-miR-574-5p showed reduced CBR1 and E-cadherin expression and increased invasive ability. There was no significant difference in proliferation ability among the groups.

Conclusions In A549 cells, the expression of CBR1 is irrelevant to cell proliferation ability but affects invasive ability; hsa-miR-574-5p can enhance the invasive ability of cells by down regulating CBR1 expression; hsa-miR-574-5p may not only be biological marker for diagnosis, but also may be an important prognostic index for patients with non-small cell lung cancer.

Keywords

A549 cells, carbonyl reductase 1, hsa-miR-574-5p, invasive ability, non-small cell lung cancer

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