Abstract
Introduction Extended spectrum ?-lactamases (ESBL) producing gram negative bacteria (GNB) have spread ominously worldwide particularly Escherichia coli (E. coli) and Klebsiella pneumonia (K. pneumoniae). Hospital outbreaks of multidrug resistant K. pneumoniae and E. coli are often caused by a new type of strain, the ESBL producers. The present study
was undertaken to detect the prevalence of E. coli and K. pneumoniae with reference
to CTX-M-15 ESBL.
Methods Identification and confirmation of E. coli and K. pneumoniae were done by standard conventional methods. Antibiotic susceptibility testing was done by Kirby Bauer’s disc diffusion technique and interpretations of susceptibility were done according to the CLSI (Clinical Laboratory Standard Institute) 2008 guidelines. Genotypic characterization of CTX-M-15 were done by polymerase chain reaction.
Results Total of 91 E. coli and 79 K. pneumoniae were isolated. Amongst all E. coli isolates, 47 (51.64%) isolates were ESBL producers while 8 (8.79%) were MBL producers. Maximum ESBL producers E. coli were from patients admitted in the ICU. Amongst all K. pneumonia, 64.55% strains were ESBL producers while 10.12% strains were MBL producers. 61 strains were detected as CTX-M-15 ?-lactamases producing ESBL. Of the total 61, 26 (42.62%) were E. coli and 35 (57.37%) were K. pneumonia.
Conclusions This study highlights the need for monitoring the spread of this multidrug resistant clonal complex throughout the nation and provides better
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