Journal of Pharmaceutical and Biomedical Sciences

Login
Select Language
Subscription Login to verify subscription
Give a gift subscription
Article Tools
Print this article
Indexing metadata
How to cite item
Finding References
Review policy
Email this article (Login required)
Email the author(Login required)
Post a Comment (Login required)

Detarium microcarpum Stem Bark Extracts Induce Apoptosis in Human Breast Adenocarcinoma MDA MB 231 Cells via cJNK Activation and Mitochondrial Cytochrome C Release

ZK Mohammed, D Templeton, Hajja Gana Hamza, A Gidado, IM Hussaini

Abstract


Detarium microcarpum stem bark extract (DMSE) is one of the medicinal herbs used by the traditional medical practitioners in North East Nigeria to treat or manage various ailments such as wounds, inflammation and some form of malignancy. This study is an attempt to assess the anti-tumor activity of DMSE on human breast adenocarcinoma MDA MB 231 cells and the underlying mechanism involved in the anti-cancer activity. The survival rate of MDA MB 231 cells was reduced in a dose-dependent manner as assessed by MTT assay. Mitogen-activated protein kinase (cJunK) was phosphorylated shortly 1 h after treatment. The translocation of mitochondrial cytochrome C to the cytosol was observed after 3 h post-treatment. In conclusion, these results suggest that DMSE possibly causes mitochondrial damage leading to cytochrome release in cytosol and the activation of c-JUN kinase resulting in apoptosis of human breast adenocarcinoma MDA MB 231 cells.

Keywords


Deterium microcarpum, stem bark extract, apoptosis

Full Text:

References


Dhillon AS, Hagan S, Rath O, Kolch W. MAP kinase signalling pathways in cancer. Oncogene. 2007;26:3279–90.

Ebi GC, Afieroho OE. Phytochemical analysis and antimicrobial studies on D. microcarpum (Guill and Sperr) Seeds. Afr J Biotech.2011;10:10457–62.

Gogvadze V, Orrenius S, Zhivotovsky B. Multiple pathways of cytochrome c release from mitochondria in apoptosis. Biochim Biophys Acta. 2006;1757:639–47.

Goldstein JC, Muñoz-Pinedo C, Ricci JE, Adams SR, Kelekar A, Schuler M, et al. Cytochrome c is released in a single step during apotosis. Cell Death Differ. 2005;12:453–62.

Hamza HG, Saleh AI, Mohammed ZK, Ngadda HA. Effect of aqueous extract of Detarium microcarpum (Guill & Sperr) on mycotoxin-induced tissue damage in albino rats. J Pharm Biomed Sci. 2014;04:92–99.

Hamza-Idris H, Oyeanusi SA. In vitro evaluation of stembark extract of Detarium microcarpum (Guill & Sperr) for antimicrobial activity. Nig J Exp Appl Biol. 2012;13(1):55–59.

Li CJ, Wang C, Pardee AB. Induction of apoptosis by beta-lapachone in human prostate cancer cells. Cancer Res. 1995;55:3712–15.

Mohammed ZK, Daja M, Hamza HG, Gidado A, Hussaini IM. Ethnomedicinal survey of folkloric plants used in managing breast cancers by the traditional medical practitioners of North-East Nigeria. J Med Appl Bioscience. 2014;6(1):29–43.

Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65: 55–63.

Musílková J, Kovár J. Additive stimulatory effect of extracellular calcium and potassium on non-transferrin ferric iron uptake by HeLa and K562 cells. Biochim Biophys Acta. 2001;1514:117–26.

Newmeyer DD, Ferguson-Miller S. Mitochondria: releasing power for life and unleashing the machineries of death. Cell.2003;112:481–90.

Olugbuyiro JAO, Moody JO, Hamann MT. Inhibitory activity of Detarium microcarpum extract against hepatitis C virus Afr J Biomed Res. 2009;12(2):146–51.

Planchon SM, Wuerzberger S, Frydman B, Witiak DT, Hutson P, Church DR, et al. Beta-lapachone-mediated apoptosis in human promyelocytic leukemia (HL-60) and human prostate cancer cells: a p53 independent response. Cancer Res. 1995;55:3706–11.

Sumitra C, Krunal N. In vitro and in vivo methods for anticancer activity evaluation and some indian medicinal plants possessing anticancer properties: an overview. J Pharmacog Phytochem. 2013;2;140–52.

Li YZ, Li CJ, Pinto AV, Pardee AB. Release of mitochondrial cytochrome c in both apoptosis and necrosis induced by 8-lapachone in human carcinoma cells. Mol Med. 1999;232–39.

Xing C, Yuping D, Jing N, Xinxin Z, Xiaodong Q, Yuxin W, et al. Brevilin A, a novel natural product, inhibits janus kinase activity and blocks STAT3 signaling in cancer cells. PLOS One. 2013;8;e63697.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Journal of Pharmaceutical and Biomedical Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.