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In vitro Antifungal Activity of Pluronic-based Thermo-Sensitive Itraconazole Gels for Vaginal Administration

Ye Qiumian, Ye Xiaocui, Luo Rui, Ou Jinlai, Xu Zhenxia, Zhao Wen, Li Sha

Abstract


Vulvovaginal candidiasis (VVC) is a common genital disease disturbing women. ITZ is widely used for the treatment of VVC by oral administration in clinic. Due to its irregular absorption and systematic side effects, local administration of ITZ may be a preferred alternative for VVC treatment. The aim of this study was to investigate the in vitro antifungal activity of pluronic-based thermo-sensitive gels (TSG) of itraconazole (ITZ) for vaginal administration. 3-(4,5-2-yl)-2,5-diphenyltetrazolium bromide method was used to evaluate the antifungal activity of the ITZ-TSG against major microorganisms inducing vulvovaginal candidosis. The gelation temperature of ITZ-TSG was 25 and 35°C before and after the dilution of simulated vaginal fluid. Compared with ITZ, ITZ-TSG demonstrated effective antifungal activity and the gel vehicle showed no action. It suggested that ITZ-TSG may be a good candidate for vulvovaginal candidosis treatment.

Keywords


itraconazole, thermosentitive gels, antifungal activity in vitro, vaginal administration

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