Abstract
Objective The present study was undertaken to identify and quantify the presence of mast cells in human periodontal disease using histochemical (toluidine blue [TB]) technique.
Materials and Methods A total of 45 tissue samples were obtained for the study: GROUP 1, 15 cases of clinically healthy gingival tissues, GROUP 2, 15 cases of pericoronitis and GROUP 3, 15 cases of chronic periodontitis were selected. Periodontally healthy tissue samples were obtained from premolar teeth extracted for orthodontic reasons. Samples of chronic periodontitis were obtained from teeth extracted due to poor periodontal prognosis. In pericoronitis group impacted third molar and operculum with inflammatory signs were selected. Sample fixed in 10% buffered formalin and stained with TB stain and observed under binocular microscope.
Result Mast cell densities (cells per mm2) were significantly increased in chronic periodontitis, and pericoronitis group compared to clinically healthy gingival tissues by histochemical technique.
Conclusion In human periodontal disease there is an increase in the number of mast cells that may be contributing either in the destructive events or in the defense mechanism of periodontal disease via secretion of cytokines, cellular migration and healing processes.
Keywords
References
Surena V, Fahimeh R, Sepideh E, Nazemisalman B. Relationship between mast cell counts and different types of periodontitis.J Periodontol Implant Dent. 2010;2:56–60.
Batista AC, Rodini CO, Lara VS. Quantification of mast cells in different stages of human periodontal disease. Oral Dis.2005;11:249–54.
Carranza FA Jr, Cabrini RL. Mast cells in human gingival. Oral Surg Oral Med Oral Pathol. 1955;8:1093–9.
Forsythe P, Befus AD. Inhibition of calpain is a component of nitric-oxide induced down-regulation of human mast cell adhesion. J Immunol. 2003;170:287–93.
Villa I, Skokos D, Tkaczyk C, Peronet R, David B, Huerre M, et al. Capacity of mouse mast cells to prime T cells and to induce specific antibody responses in vivo. Immunology. 2001;102:165–72.
Welle M. Development, significance, and heterogeneity of mast cells with particular regard to the mast cell specific proteases chymase and tryptase. J Leukoc Biol. 1997;61:233–45.
Myint M, Steinsvoll S, Yaun ZN, Johne B, Helgeland K, Schenck K. Highly increased numbers of leukocytes in inflamed gingival from patients with HIV infection. AIDS. 2002;16:235–43.
Steinsvoll S, Halstensen TS, Schenck K. Extensive expression of TGF-beta1 in chronically-inflamed periodontal tissue. J Clin Periodontol. 1999;26:366–73.
Odden K, Schenck K and Hurlen B. High numbers of T cells in gingiva from patients with human immunodeficiency virus (HIV) infection. J Oral Pathol Med. 1995;24:413–19.
Myint M, Odden K, Schreurs O, Halstensen TS, Schenck K. The gingival plasma cell infiltrate in HIV-positive patients with periodontitis is disorganized. Clin Periodontol. 1999;26: 358–65.
Echtenacher B, Männel DN, Hültner L. Critical protective role of mast cells in a model of acute septic peritonitis. Nature. 1996 May 2;381(6577):75–7.
Malaviya R, Ikeda T, Ross E, Abraham SN. Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-alpha. Nature. 1996 May 2;381(6577):77–80.
Malaviya R, Abraham SN. Role of mast cell leukotrienes in neutrophil recruitment and bacterial clearance in infectious peritonitis. J Leukoc Biol. 2000 Jun;67(6):841–6.
Huttunen M, Aalto ML, Harvima RJ, Horsmanheimo M Harvima IT. Alterations in mast cells showing tryptase and chymase activity in epithelializating and chronic wounds. Exp Dermatol. 2000;9(4):258–65.
Kennett CN, Stephen W, Eley BM, Osman IA. Comparative histochemical and biochemical studies of mast cell tryptase in human gingiva. J Periodontol. 1993;64:870–77.
Kabashima H, Nagata K, Maeda K, Iijima T. Involvement of substance P, mast cells, TNF-? and ICAM-1 in the infiltration of inflammatory cells in human periapical granulomas. J Oral Pathol Med. 2002;31:775–80.
Jeffcoat MK, Williams RC, Johnson HG, Wechter WJ, Goldhaber P. Treatment of periodontal disease in beagles with lodoxamide ethyl, an inhibitor of mast cell release. J Periodontal Res. 1985; 20:532–41.
Næsse EP, Schreurs O, Helgeland K, Schenck K, Steinsvoll S. Matrix metalloproteinases and their inhibitors in gingival mast cells in persons with and without human immunodeficiency virus infection. J Periodontal Res. 2003;38:575–82.
Günhan M, Bostanci H, Gunhan O, Demiriz M. Mast cells in periodontal disease. Ann Dentistry. 1991;50:25–9.
Kennett CN, Cox SW, Eley BM. Investigations into the cellular contribution to host tissue proteases and inhibitors in gingival crevicular fluid. J Clin Periodontol. 1997;24:424–31.
Walsh LJ. Mast cells and oral inflammation. Crit Rev Oral Biol Med. 2003;14:188–98.
Steinsvoll S, Helgeland K, Schenck K. Mast cells – a role in periodontal diseases? J Clin Periodontol. 2004;31:413–9.
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Journal of Pharmaceutical and Biomedical Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.