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Overexpressing Melanopsin Protects Retinal Ganglion cells against N-methyl-D-aspartate-Induced Excitotoxicity

Xin Ren, Xiaopeng Luo, Xinnan Li, Zhiqiang Xiao, Yongxia Huang, Hui Yang

Abstract


Aim: To investigate whether the expression of melanopsin (opsin 4, Opn4) can protect retinal ganglion cells (RGCs) against retinal injury induced by N-methyl-D-aspartate (NMDA).

Methods: Sprague-Dawley rats were intravitreally injected with AAV2-CMV-Opn4-IRES-ZsGreen or AAV2 –CMV-IRES-ZsGreen as the control. Then 3 weeks after the virus injection, rats received intravitreal injections of NMDA to induce the apoptosis of RGCs. Then after 5 days, the survival rates of RGCs were detected by immunofluorescence staining. Protective effect of melanopsin was further evaluated by transfecting HEK293 cells with melanopsin or control virus, and then examining the viability of HEK293 cells after serum deprivation by flow cytometry.

Results: Melanopsin was specifically transfected into RGCs by intravitreal injection of virus carrying melanopsin. Overexpressing melanopsin increased the density of RGC after NMDA injury compared with the control virus. Overexpressing melanopsin in HEK293 cells also improved the viability of cells after serum deprivation.

Keywords


Melanopsin; NMDA; retinal ganglion cells.

Full Text:

References


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