Abstract
Purpose To compare the antibacterial efficacy of three bonding agents, ADPER EASY BOND, ONE COAT 7.0, PALFIQUE BOND against Streptococcus mutans.
Materials and Methods The three seventh generation bonding systems: ADPER EASY BOND, ONE COAT 7.0 and PALFIQUE BOND were taken. A total of 3.8 g/100 mL of Mueller Hinton Agar (MHA) was autoclaved at 110°C for 15 min. Media was then poured in petri plates and solidified at room temperature. After solidification, 3 wells were punched with hollow tube under sterilized conditions. Well 1 as CHX, well 2 as Normal saline (NS) and well 3 A/B/C compounds. About 5 mL of seed culture of S. mutans strain MTCC no. 497 was prepared in Mueller Hinton broth. About 6 ?L of CHX (+ve control) and NS (?ve control) was added to wells 1 and 2 in each plate. About 100 ?L of seed inoculum was spread on each MHA plates. For each bonding agent 10 replicas of experiment was set at room temperature. A total of 6 ?L of bonding agent A, B and C were added to well
3 respectively in each set of experiment. To study zone of inhibition, plates were incubated at 37°C for 12 h.
Conclusion Different bonding systems possess different degrees of antibacterial activity. Seventh generation bonding agent of compounds A and C do not have antibacterial action while compound B has.
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