Improvement of in vitro Anticancer Activity of Doxorubicin by Sodium Alginate Nanoparticles Delivery

Jianyuan Kuang; Qiumian Ye; Tian Gong; Yangyang Chen; Sha Li  | Vol 08 | Issue 05 | Pp:89-93.

Improvement of in vitro Anticancer Activity of Doxorubicin by Sodium Alginate Nanoparticles Delivery

Jianyuan Kuang, Qiumian Ye, Tian Gong, Yangyang Chen, Sha Li | Vol 08 | Issue 05 | Pp:89-93.

Abstract

Nanoparticles (NPs) have been widely applied in drug delivery of anticancer drugs to reduce their toxicity to normal tissue. Sodium alginate nanoparticles (ALG-NPs) were prepared in our previous study to deliver doxorubicin (DOX) to expect to increase the efficacy of DOX, thus to decrease the dosage and lessen the toxicity. The cytotoxicity of blank ALG-NPs and DOX loading ALG-NPs (DOX–ALG-NPs) were investigated in Hela, MCF-7, MDA-MB-231, A549 and NCI-H1299 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide staining method. When the concentration of ALG-NPs was not
more than 125 ?g/mL, ALG-NPs generally had no inhibition to the growth of cancer cells with a cell viability higher than 90%. DOX–ALG-NPs demonstrated stronger anticancer activity than free DOX solution at most tested concentrations after 24, 48 and 72 h treatment in the tested cell lines. The IC50 value of DOX–ALG-NPs was less than DOX solution after different treatment time in different tested cells. Higher intracellular uptake of DOX was observed in DOX–ALG-NPs group than in free DOX solution group under inverted fluorescence microscope. The results indicated that DOX–ALG-NPs might achieved the
required anticancer efficacy at low concentration, thus to be possible to reduce the toxic and side effect of drug.

Keywords

sodium alginate nanoparticles, doxorubicin, anticancer activity in vitro

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