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Preparation of Doxorubicin-loading Sodium Alginate Nanoparticles and its Anticancer Activity in HepG2 cells

Yangyang Chen, Qiumian Ye, Tian Gong, Jianyuan Kuang, Sha Li | Vol 08 | Issue 05 | Pp:79-83.

Abstract


Sodium alginate (ALG) is a widely used natural polysaccharide in microparticle drug delivery system. In this work, we prepared blank sodium alginate nanoparticles (ALG-NPs) by emulsification followed by ionotropic gelation as reported previously. Doxorubicin (DOX) was adsorbed in ALG-NPs to yield DOX-loading sodium alginate nanoparticles (DOX–ALG-NPs). The shape, size, charging property, drug-loading capacity and drug release behavior of DOX–ALG-NPs was characterized. The spherical DOX–ALG-NPs were about 270 nm with an entrapment efficiency (EE) higher than 94% and a drug loading rate (DL) higher than 19%. DOX–ALG-NPs showed a sustained and complete release in normal saline (NS), phosphate buffer solution of pH 6.8 and 7.4 within 72 h. DOX–ALGNPs showed distinct cytotoxicity in HepG2 cells. The inhibitive rate of DOX–ALG-NPs was significantly higher than that of DOX when drug concentration was higher than 1 microgram/mL. The uptake of DOX in DOX–ALG-NPs group was obviously more than that in DOX solution group. The potential anticancer activity of DOX–ALG-NPs may be due to the high uptake of NPs by tumor cells.

Keywords


sodium alginate, nanoparticles, doxorubicin, preparation, anticancer activity, HepG2 cells

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