Spinal Cord Injury Causes Infection of the Spinal Cord, Liver, and Lung in Mice

Xiaoya Tang; Abdikarin Mohamed Artan; Yiwen Ruan | Vol 08 | Issue 04 | Pp:63-65.

Spinal Cord Injury Causes Infection of the Spinal Cord, Liver, and Lung in Mice

Xiaoya Tang, Abdikarin Mohamed Artan, Yiwen Ruan | Vol 08 | Issue 04 | Pp:63-65.

Abstract

Objective To investigate whether spinal cord injury (SCI) will cause bacteria transfer to other organs and induce these organs infection in mice.

Methods SPF C57BL/6J mice were randomly divided into two groups: SCI group (n = 6) and sham operation group (n = 6). Mice were anesthetized with 1.25% tribromoethanol and performed with a moderate contusion (0.5 mm/0.4 s) injury at the spinal cord T10 with an LSA-Vibraknife. One week after the operation, the spinal cord, liver, and lung tissues in each
group of mice were collected for detection of the bacterial DNA using polymerase chain
reaction (PCR) to determine.

Results It turns out that positive 16s rRNA bands were detected in the spinal cord, the liver, and lungs after spinal cord injury. However, no positive bands were measured in the sham group.

Conclusion The infection of the liver and the lung results suggest that the bacteria of the intestine may enter the blood stream after SCI and cause the infection in these two organs. While spinal cord infection may cause from the injury itself or from translocation of intestinal bacteria.

Keywords

spinal cord injury, 16s rRNA, bacterial translocation, liver, lung

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References

Biering-Sørensen F, Brown DJ, Officer A, Shakespeare T, von Groote P, Wyndaele JJ. IPSCI: a WHO and ISCoS collaboration report. Spinal Cord. 2014;52:87.

Boekamp JR, Overholser JC, Schubert DS. Depression following a spinal cord injury. Int J Psychiatry Med. 1996;26:329–349.

Enck P, Greving I, Klosterhalfen S, Wietek B. Upper and lower gastrointestinal motor and sensory dysfunction after human spinal cord injury. Prog Brain Res. 2006;152:373–384.

Macfie J. Current status of bacterial translocation as a cause of surgical sepsis. Br Med Bulletin. 2004;71:1–11.

Elaine YH, Sara WM, Hsien S, Sharon G, Embriette RH, T McCue T, et al. Microbiota modulate behavioral and physiological abnormalities associated with neurodevelopmental disorders. Cell. 2013;155:1451–1463.

Zhang HT, Gao ZY, Chen YZ, Wang TH. Temporal changes in the level of neurotrophins in the spinal cord and associated precentral gyrus following spinal hemisection in adult Rhesus monkeys. J Chem Neuroanatomy. 2008;36:138–143.

Kigerl KA, Hall JC, Wang L, Mo X, Yu Z, Popovich PG. Gut dysbiosis impairs recovery after spinal cord injury. J Exp Med.2016;213:2603–2620.

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