Functional Proteomic Studies of Lycorine-treated MDCK Cells on Highly Pathogenic Avian Influenza H5N1 Virus Infection

Hui Bin Huang; Jun He; Zhao Yue Zhang; Yao Lan Li

Functional Proteomic Studies of Lycorine-treated MDCK Cells on Highly Pathogenic Avian Influenza H5N1 Virus Infection

Hui Bin Huang, Jun He, Zhao Yue Zhang, Yao Lan Li

Abstract

Stable isotope labeling with amino acids in cell culture (SILAC), a proteomic method, has been used to analyze the protein level variation of influenza virus-infected host cells treated by lycocine. In total, we identified 176 cellular proteins in M/L group and 188 proteins in H/M group. Sixty-three proteins were identified with an upward or downward trend in M/L and M/H group. We employed bioinformatic approaches including functional clustering, gene ontology, and pathway (KEGG) enrichment tests to uncover co-regulated cellular protein sets, assigned the individual subsets to their biological functions, and determined their interrelation within the progression of viral infection. To our knowledge, this is the first time to describe dynamic changes of the cellular and, of note, the viral proteomes in a time dependent manner simultaneously. Through cluster analysis, time-dependent patterns of protein abundances revealed highly dynamic up- and/or down-regulation processes. Taken together, our study showed strong evidence that virus infection has a major impact on the cell status at the protein level. In conclusion, we provide the first comprehensive protein profile characterization of active compound lycorine effects, and provide evidence that PCNA represent potential therapeutic targets for more effective treatment of influenza infections.

Keywords

SILAC, H5N1 influenza virus, PCNA, lycorine

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