Abstract
Methods C57BL/6 mice were divided into control group, model group, matrine groups (including 80 mg/kg, 40 mg/kg and 20 mg/kg dose groups), and dexamethasone acetate group (positive control). The sensitizer was first applied on the mice abdomen for sensitization and then on the cheek for challenge to elicit ACD. We confirmed the antipruritic and analgesic effects of matrine by comparing the spontaneous scratching and wiping directed to the cheek as well as the changes of cheekfold thickness in the different groups of mice.
Results The scratching of model group was increased significantly when it compared with the control group (P < 0.05). Compared to the model group, the scratching of each matrine group decreased significantly (P < 0.05); The wiping of model group was increased significantly when it compared to control group (P < 0.01). Compared to the model group, the wiping of each matrine group decreased significantly (P < 0.01); The changes of skin fold thickness of model group increased significantly when it compared to the control group (P < 0.01). Compared to model group, the changes of the skin fold thickness of each matrine group decreased significantly (P < 0.01).
Conclusion We have successfully established an ACD model that provides a behavioral differentiation by itch-like scratching and pain-like wiping in mice. Results proved that matrine can obviously improve the itch and pain sensations of ACD mice, and the effects of matrine has a dose-dependent manner obviously.
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References
Yosipovitch G, Greaves MW, Schmelz M. Itch. Lancet.2003;361:690–694.
Mathias CG. Contact dermatitis and workers’ compensation: criteria for establishing occupational causation and aggravation. J Am Acad Dermatol. 1989;20:842–848.
Peng C, Jinhong H, Quangang Z, et al. Studies of effective constituent and compatibility mechanism of prescription of Sophorae Flavesentis Radix. Chinese herbal medicine. 2008;11:1631–1635.
Minge J, Wenji Sun. Conversion of oxymatrine and matrine in sophorae flavesentis radix and the prescription of sophorae flavesentis radix. Chinese Journal of Pharmaceutical Analysis. 2003;90–93.
Peiser M, Tralau T, Heidler J, Api AM, Arts JH, Basketter DA, et al. Allergic contact dermatitis: epidemiology, molecular mechanisms, in vitro methods and regulatory aspects. Current knowledge assembled at an international workshop at BfR, Germany. Cell Mol Life Sci. 2012;69:763–781.
Dev S, Mizuguchi H, Das AK, Maeyama K, Horinaga S, Kato S, et al. Kujin suppresses histamine signaling at the transcriptional level in toluene 2,4-diisocyanate-sensitized rats. J Pharmacol Sci. 2009;109:606–617.
Minglian Y, Jianli M, Liang z, et al. Pharmacodynamics study of oxymatrine anti-pruritus emulsifiable paste. China Pharmacist. 2008;18–20.
Yamaguchi-Miyamoto T, Kawasuji T, Kuraishi Y, Suzuki H. Antipruritic effects of Sophora flavescens on acute and chronic itch-related responses in mice. Biol Pharm Bull. 2003;26:722–724.
Gong SS, Li YX, Zhang MT, Du J, Ma PS, Yao WX, et al. europrotective effect of matrine in mouse model of vincristine-induced neuropathic pain. Neurochem Res. 2016;41:3147–3159.
Haiyan W, Yuxiang L, Linglu D, Tingting X, Yinju H, Hongyan L, et al. Antinociceptive effects of matrine on neuropathic pain induced by chronic constriction injury. Pharm Biol. 2013;51:844–850.
Happle R, Kalveram KJ, Büchner U, Echternacht-Happle K, Göggelmann W, Summer KH. Contact allergy as a therapeutic tool for alopecia areata: application of squaric acid dibutylester. Dermatologica.1980;161:289–297.
Scott AE, Kashon ML, Yucesoy B, Luster MI, Tinkle SS. Insights into the quantitative relationship between sensitization and challenge for allergic contact dermatitis reactions. Toxicol Appl
Pharmacol. 2002;183:66–70.
Camouse MM, Swick AR, Ryan CA, Hulette B, Gerberick F, Tinkle SS, et al. Determination of in vivo dose response and allergen-specific T cells in subjects contact-sensitized to squaric acid dibutyl ester. Dermatitis. 2008;19:95–99.
Christensen AD, Haase C. Immunological mechanisms of contact hypersensitivity in mice. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2012;120:1–27.
Gaspari AA, Katz SI. Contact hypersensitivity. Curr Protocol Immunol. 2001; Chapter 4: Unit 4 2.
Weltzien HU, Moulon C, Martin S, Padovan E, Hartmann U, Kohler J. T cell immune responses to haptens. Structural models for allergic and autoimmune reactions. Toxicology. 1996;107:141–151.
Vocanson M, Hennino A, Rozières A, Poyet G, Nicolas JF. Effector and regulatory mechanisms in allergic contact dermatitis. Allergy. 2009; 64:1699–1714.
Nagano T, Katase M, Tsumura K, Saito M, Matsuda T. Inhibitory effects of dietary soyasaponin on 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice. Exp Dermatol. 2017;26:249–254.
Hu YE, Dai SF, Liu Y, et al. Study on the therapeutic mechanisms of pseudolaric acid in mice with allergic contact dermatitis. Asian Pacific J Trop Med. 2016;9:668–671.
Pall PS, Hurwitz OE, King BA, LaMotte RH. Psychophysical measurements of itch and nociceptive sensations in an experimental model of allergic contact dermatitis. J Pain. 2015;16:741–749.
Qu L, Fan N, Ma C, et al. Enhanced excitability of MRGPRA3- and MRGPRD-positive nociceptors in a model of inflammatory itch and pain. Brain. 2014;137:1039–1050. Shimada SG, LaMotte RH. Behavioral differentiation between itch and pain in mouse. Pain. 2008;139:681–687.
Ishiuji Y, Coghill RC, Patel TS, Oshiro Y, Kraft RA, Yosipovitch G. Distinct patterns of brain activity evoked by histamine-induced itch reveal an association with itch intensity and disease severity in atopic dermatitis. Br J Dermatol. 2009;161:1072–1080.
Ikoma A, Steinhoff M, Stander S, Yosipovitch G, Schmelz M. The neurobiology of itch. Nat Rev Neurosci. 2006;7:535–547.
McCracken LM, Carson JW, Eccleston C, Keefe FJ. Acceptance and change in the context of chronic pain. Pain. 2004;109:4–7.
Zhang J WW, Duan Z. Progress of research and application of matrine-type alkaloids. Progress Modern Biomed. 2007;7:445–454.
Li T, Wong VK, Yi XQ, Wong YF, Zhou H, Liu L. Matrine induces cell anergy in human Jurkat T cells through modulation of mitogen-activated protein kinases and nuclear factor of activated T-cells signaling with concomitant up-regulation of anergy-associated genes expression. Biol Pharm Bull. 2010;33:40–46.
Liu JY, Hu JH, Zhu QG, Li FQ, Wang J, Sun HJ. Effect of matrine on the expression of substance P receptor and inflammatory cytokines production in human skin keratinocytes and fibroblasts. Int Immunopharmacol. 2007;7:816–823.
Santos AR, Calixto JB. Ruthenium red and capsazepine antinociceptive effect in formalin and capsaicin models of pain in mice. Neurosci Lett. 1997;235:73–76.
Kwak JY, Jung JY, Hwang SW, Lee WT, Oh U. A capsaicin-receptor antagonist, capsazepine, reduces inflammation-induced hyperalgesic responses in the rat: evidence for an endogenous capsaicin-like substance. Neuroscience. 1998;86:619–626.
Cevikbas F, Wang X, Akiyama T, Kempkes C, Savinko T, Antal A, et al. A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1. J Aller Clin Immunol. 2014;133:448–460.
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