Action of Root-Endophytic Fungi of Tinospora cordifolia (Willd.) Miers against Cancer Cell Lines and Human Pathogens

Thangaraj A; Mythili M; Mujeera Fathima

Action of Root-Endophytic Fungi of Tinospora cordifolia (Willd.) Miers against Cancer Cell Lines and Human Pathogens

Thangaraj A, Mythili M, Mujeera Fathima

Abstract

The aim of the study was to isolate and identify the promising antibacterial and anticancer metabolites produced by fungal endophytes found in the roots (underground roots) of Tinospora cordifolia. Plant endophytic fungi are the important, novel resource of natural bioactive metabolites with their potential applications in various industries including medicine. Underground roots of healthy T. cordifolia were collected and the fungal endophytes were isolated using Luria-Bertani agar medium at standard conditions. Pure cultures were raised and identified on the basis of the colonial morphology and the characteristics of fungal spores. Considering the importance of this plant, antibacterial activity was assayed using four human pathogenic bacteria in bioactive metabolites. After screening, MTT assay was performed in the crude extract. The results show that although significant antibacterial activities were found in the crude extract against human pathogens, no appreciable anticancer activity was found in MCF-7 cell line.

Keywords

Tinospora cordifolia (willd.)miers, root endophytes, phytochemicals, cytotoxicity, MTT assay, crude extract, antibacterial, anticancer

Full Text:

References

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. Internationale

Pharmaceutica Sciencia. 2011;1:98–106.

Okwu DE. Evaluation of the chemical composition of indigenous spices and flavouring agents. Global J Pure and Appl Sci.2001;7:455–459.

Soham S, Shyamasree G. Tinospora cordifolia: One plant, many roles. Anc Sci Life. 2012;31:151–159.

Sangeetha MK, Raghavendran BHR, Gayathri V, Vasanthi HR. Tinospora cordifolia attenuates oxidative stress and distorted carbohydrate metabolism in experimentally induced type 2 diabetes in rats. J Nat Med. 2011;65:544–550.

Sudha P, Zinjarde SS, Bhargava SY, Kumar AR. Potent ?-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complement Altern Med. 2011;11:5.

Umamaheswari S, Mainzen Prince PS. Anti hyperglycaemic effect of ‘Ilogen-Excel’, an ayurvedic herbal formulation in streptozotocin-induced diabetes mellitus. Acta Pol Pharm. 2007;64:53–61.

Mainzen SPP, Menon VP. Hypoglycaemic and hypolipidaemic action of alcohol extract of Tinospora cordifolia roots in chemical induced diabetes in rats. Phytother Res. 2003;17:410–413.

Prince PS, Kamalakkannan N, Menon VP. Restoration of antioxidants by ethanolic Tinospora cordifolia in alloxan-induced diabetic Wistar rats. Acta Pol Pharm. 2004;61:283–287.

Stanely P, Prince M, Menon VP. Hypoglycaemic and other related actions of Tinospora cordifolia roots in alloxan-induced diabetic rats. J Ethnopharmacol. 2000;70:9–15.

Gupta R, Sharma V. Ameliorative effects of Tinospora cordifolia root extract on histopathological and biochemical changes induced by aflatoxin-b (1) in mice kidney. Toxicol Int. 2011;18:94–98.

Hamsa TP, Kuttan G. Tinospora cordifolia ameliorates urotoxic effect of cyclophosphamide by modulating GSH and cytokine levels. Exp Toxicol Pathol. 2012;64:307–314.

Ganesh CJ, Rao SK. Evaluation of cytotoxic effects of dichloromethane extract of Guduchi (Tinospora cordifolia Miers ex Hook F &THOMS) on cultured HeLa cells. Evid Based Complement Alternat Med. 2006;3:267–272.

Rao SK, Rao PS. Alteration in the radiosensitivity of HeLa cells by dichloromethane extract of guduchi (Tinospora cordifolia). Integr Cancer Ther. 2010;9:378–384.

Kapur P, Pereira BM, Wuttke W, Jarry H. Androgenic action of Tinospora cordifolia ethanolic extract in prostate cancer cell line LNCaP. Phytomedicine. 2009;16:679–682.

Jagetia GC, Rao SK. Evaluation of cytotoxic effects of dichloromethane extract of Guduchi (Tinospora cordifolia Miers ex Hook F &Thoms) on cultured HeLa cells. Evid Based Complement Alternat Med. 2006;3:267–272.

Rao SK, Rao PS, Rao BN. Preliminary investigation of the radiosensitizing activity of guduchi (Tinospora cordifolia) in tumor-bearing mice. Phytother Res. 2008;22:1482–1489.

Johannes H, Gabriele B, Barbara S. Isolation Procedures for Endophytic Microorganisms. Springer, Berlin, 2006;p. 299.

Tan RX, Zou WX. Endophytes: a rich source of functional metabolites. Nat Prod Rep. 2001;18:448–459.

Harborne JB. Phytochemistry. Academic Press, London, 1993; pp: 89–131.

Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH. 1995. 7th ed. Washington: ASM; Manual of Clinical Microbiology, 1995; pp: 1773.

Gorajana A, Venkatesan M, Vinjamuri S, Kurada BV, Peela S, Jangam P, et al. Resistoflavin, cytotoxic compound from marine actinomycetes, Streptomyces chibaenis AUBN1/7. Microbiol Res. 2007;162:322–327.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxici

ty assays. J Immunol Methods. 1983;65:55–63.

Jagetia GC, Rao SK. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in Ehrlich ascites carcinoma bearing mice. Biol Pharm Bull. 2006;29:460–466.

Silva G, Teles H, Trevisan H, Bolzani V, Young MCM, Pfenning L, et al. New bioactive metabolites produced by Phomopsis cassia, an endophytic fungus in Cassia spectabilis. J Brazilian Chem Soc.

;16:1463–1466.

Mahesh B, Satish S. Antimicrobial activity of some important medicinal plants against plant and human pathogens. World J Ag Sci. 2008;4(S):839–843.

Kakkar DR, Verma A. Antibacterial activity of Tinospora cordifolia. J Glob Pharma Tech. 2011;3:8–12.

Kawsar H, Hossai A, Uddin H. Antimicrobial and cytotoxic activity of Tinospora cardifolia (Family:Menispermaceae). Int J Pharma Sci Res. 2011;2:656–658.

Refbacks

There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Journal of Pharmaceutical and Biomedical Sciences