Abstract
Aim The aim of this study was to evaluate the effects of CA ethanolic extract encapsulated in chitosan nanoparticles (CAEE + CNP) on skin cell proliferation, collagen synthesis and AQP3 expression in vitro, when compared to RA.
Methods Microculture tetrazolium assay was conducted to analyse the proliferation of normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK) at 24, 48 and 72 h. Type I and III collagen synthesis was evaluated at the same time points using ELISA. Aquaporin 3 expression at 24 h was evaluated using immunocytochemistry and measured quantitatively using ImageJ software. All treatments involved several concentrations of CAEE + CNP and RA through serial dilution.
Results Collagen type I and III synthesis of NHDF and NHEK was neither significantly different from untreated controls nor from RA-treated cells. Nevertheless, CAEE + CNP stimulated the proliferation of both NHDF and NHEK. Additionally, AQP3 expression in both cell types was upregulated by CAEE + CNP.
Conclusion CAEE + CNP is a promising formulation for anti-aging activity by inducing skin cell proliferation and AQP3 expression. The clinical trials are still needed to evaluate skin hydration in vivo
Keywords
References
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