Evaluation of Parkinson’s disease in 3 rat models induced by three different unilateral injections of 6-hydroxydopamine

Kun Zhang; Jiayu Gao; Zaijun Zhang

Evaluation of Parkinson’s disease in 3 rat models induced by three different unilateral injections of 6-hydroxydopamine

Kun Zhang, Jiayu Gao, Zaijun Zhang

Abstract

This study was designed to investigate the difference between three 6-hydroxydopamine (6-OHDA)-induced rat PD models. Those rats model were established by stereotaxic unilateral 6-OHDA injection into different parts of nigrostriatal pathway including the striatum (ST), the substantia nigra pars compacta (SNPc) and the ventral tegmental area (VTA) in left side of rat brain. To detect pathological change in the unilaterally lesion 6-OHDA rats, the intact hemisphere work as a internal control. Immunohistochemical staining was used to evaluate the expression of tyrosine hydroxylase (TH) in substantia nigra pars compacta, as indication of injure of dopaminergic neuron. Remarkable TH-postive neuron loss was found in substantia nigra pars compacta in all 6-OHDA injected groups. The steep depletion of the content of dopamine and its metabolites of in striatum was also observed in all 6-OHDA injected groups. The 6-OHDA-impaired rats demonstrate a decrease of body weight compared to sham group rats. Behavioral assessments of motor impairments in the unilateral 6-OHDA rat model were done by apomorphine-induced rotation tests. In apomorphine-induced contralateral rotations test, the ST injection group show increasing rotation from the 2th week to the 4th week and high success rate of modeling; SNpc group and VTA + SNpc groups showed stable rotations from the 2nd week but low success rate. These results suggested that ST group has higher success rate and is more practicable than the other two groups.

Keywords

6-hydroxydopamine; rat model, Parkinson’s disease; striatum; substantial nigra

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