Abstract
disorders including schizophrenia. Effect of oxidative stress has been put forward as a
plausible mechanism for this, although with variable results.
Aim To assess the effects of oxidative stress induced membrane lipid damage and cellular
protein alteration on Na+/K+-ATPase activity in newly diagnosed schizophrenia patients.
Settings and Design It was undertaken as a hospital-based horizontal, observational
case control study in a tertiary care urban hospital involving 46 cases and 50 controls
spanning a period of 1 year.
Materials and Methods RBC membrane Na+/K+-ATPase levels were measured by the
NADH/ATP coupled kinetic assay method. Serum thiobarbituric acid reacting substances
(TBARS) and protein carbonyl (PC) values were measured by spectrophotometric techniques.
Tissue protein was estimated by Lowry’s method.
Statistical Analysis After testing for normal distribution of the data obtained, independent t
test, Pearson’s correlation analysis and multiple linear regression analyses were performed to
analyse the difference between mean values, strength of association between parameters and
predictive values of oxidative stress parameters on the Na+/K+-ATPase activity, respectively.
Results Membrane Na+/K+-ATPase activity was significantly lower with significantly higher
values of serum TBARS and PC in cases (p < 0.001). Although, both PC and TBARS showed
significant negative correlation with Na+/K+-ATPase activity, it was found to be dependent
significantly on the serum TBARS values only.
Conclusion The study indicates that although, the increased oxidative stress in
schizophrenia damages both membrane lipids and tissue proteins significantly, the compromised
Na+/K+-ATPase activity is more crucially dependent on membrane lipid damage.
Keywords
References
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