Abstract
Aim: To comparatively evaluate the antimicrobial efficacy of manuka honey and pomegranate mouth wash on gram negative
periodontal pathogens of red, orange and green complex (Porphyromonas gingivalis {P.G.}, Prevotella intermedia {P.I.},
Forshythus nucleatum {F.N.}, Aggregatibacter actinomycetemcomitans {A.A.}) in in-vitro conditions.
Materials and methods: Nutrient agar plates were inoculated by rubbing sterile cotton swabs dipped into bacterial suspensions
of P. gingivalis, P. intermedia, F. nucleatum, A. actinomycetemcomitans (overnight cultures grown at 37°C on nutrient agar) over
the entire surface of the plate. After inoculation, 10 mm diameter five wells were cut into the surface of the agar using a sterile
cork borer for each sample. Honey, pomegranate mouthwash, and chlorhexidine were added into wells in four different plates
containing above mentioned four different bacterias. Plates were then incubated at 37°C for 24 hours. The diameters of zones
of inhibition were measured using a Vernier calipers on all the plates. The mean score of zones of inhibition was calculated.
Similar procedure for all different concentration was performed.
Results: 0.2% chlorhexidine had edge over the manuka honey and pomegranate mouthwash in inhibiting the growth of microorganisms. A. actinomycetemcomitans showed the greatest zone of inhibition by manuka honey of 18 mm, pomegranate
mouthwash 19 mm as compared to chlorhexidine getting 20 mm. Both were most effective against A.A. followed by F. nucleatum,
P. gingivalis, P. intermedia as compared with 0.2 % chlorhexidine.
Conclusion: Manuka honey and pomegranate mouthwash can create new horizons in the field of chemical agents that can be
used as an adjunct to mechanical periodontal therapy can be explored and compared with 0.2% chlorhexidine.
Keywords
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References
Corbet EF. Periodontal diseases in Asians. J Int Acad Periodontol 2006;8:136–44.
Corbet EF, Leung WK. Epidemiology of periodontitis in the Asia and Oceania regions. Periodontol 2000
;56:25–64.
Emani S, Gunjiganur GV, Mehta DS. Determination of the antibacterial activity of simvastatin against
periodontal pathogens, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: an in
vitro study. Contemp Clin Dent 2014;5:377–82.
Asif M. The role of fruits, vegetables, and spices in diabetes. Int J Nutr Pharmacol Neurol Dis
;1:27–35.
Vasconcelos LC, Sampaio FC, Sampaio MC, Pereira Mdo S, Higino JS, Peixoto MH. Minimum inhibitory
concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis
and C. albicans. Braz Dent J 2006;17:223–7.
Sherlock et al. Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey
against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa.
BMC Comp Alt Med 2010:10;47–51.
Toda M, Okubo S, Hiyoshi R, Shimamura T. The bactericidal activity of tea and coffee. Letters in Applied
Microbiology 1989;8:123–5.
Fardiaz S. Antimicrobial activity of coffee (Coffea robusta) extract. ASEA Food J 1995;10:103?6.
Carranza FA. In Carranza’s clinical periodontology, 9th ed. Philadelphia, USA: WB Saunders company;
Pai MB, Prashant GM, Muralikrishna KS, Shivkumar KM, Chandu GN. Antifungal efficacy
of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on candida
albicans: as an in vitro study. Indian J Dent Res 2010;21:334–36.
Socransky SS, Haffejee AD. The bacterial etiology of destructive periodontal disease: Current concepts.
J Periodontol 1992:63;322.
Takarada K. et al. A comparision of the antimicrobial efficacies of essential oils against oral pathogens.
Oral Microbiol Immunol 2004:19: 61–4.
Jenkins R, Burton N, Cooper R. Manuka honey inhibits cell division in methicillin-resistant
Staphylococcus aureus. J Antimicrob Chemother 2011;66:2536–42.
Haslam, E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action. Journal of
Natural Products 1996;59:205–15.
Adiga S, Tomar P, Rajput RR. Effect of Punica granatum peel aqueous extract on normal and dexamethasone
suppressed wound healing in wistar rats. Int J Pharmaceut Sci Rev Res 2010;5:134–40.
Sharma S, Suchetha A, Vijendra R, Bharwani A. An in vitro microbiological study evaluating the
efficacy of soluneem (a water soluble neem formulation from Azadirachta indica) against periodontopathic
microorganisms. J Oral Health Comm Dent 2012;6:4–9.
Dadawala S, Dave R, Shah M, Dave D. Comparative evaluation of antimicrobial efficacy of
manuka honey and chlorhexidine mouthwash on red , orange and green complex of periodontal
pathogens — an in vitro study. Res Rev J Dent Sci 2016;4:20–3
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