Antibacterial Activity of Allium ampeloprasum Against ESBL+ve Escherichia Coli and Methicillin-Resistant Staphylococcus Aureus by Transmission Electron Microscopy

Abstract

Infectious diseases caused by MDR pathogens are one of the primary health concerns globally, affecting millions of people every year. Infectious diseases are responsible for high morbidity throughout the world, accounting for about a quarter of deaths around the globe. This growing problem has necessitated the exploration and development of novel antimicrobials such as natural products, plant phytochemicals and fabricated antibiotics with less or no side effects. Medicinal plants are known to be valuable resources for the development of novel antimicrobial drugs. Medicinal herbs are better equipped at metabolic Bio-engineering of active compounds than conventional laboratory approaches. Preliminary antibacterial assay by agar well assay of Allium Ampeloprasum on MDR pathogens MRSA and ESBL +ve E.coli have shown significant antibacterial activity. After clear zone of inhibition formation, the further evaluation of antimicrobial activity was performed by Transmission Electron Microscopy (TEM), and morphological changes are observed at the cellular level. Ultra structure observations have demonstrated severe damage to both the bacterial cells of MRSA and ESBL+ve E.coli. For the first time, the antibacterial activity of Allium leaf extract against MRSA and ESBL+ve E.coli was confirmed by using TEM micrographs.