Journal of Agriculture Biotechnology

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Assessment Genetic Diversity Landraces In Onobrychis Sativa Using SSR, RAPD and SRAP Markers

Mohammad Sharifi Sedeh

Abstract


In order to evaluate genetic diversity cultivated sainfoin accessions collected from different regions of the world, SSR markers from medicago genus were used. Polymorphism information content value ranged from 0.2 to 0.043 with an average of 0.33. Cluster analysis based on SSR markers grouped the sainfoin population in two main groups (Iranian and exotic cultivate sainfoin) with two sub cluster for each group. In another survey was performed to find out an efficiency of ecological factors on the genetic similarity was investigated among five population of Onobrychis viccifolia East Azarbaijan, Iran using RAPD and bulked DNA, The largest and shortest genetic were detected between Bonab and Heris (0.04904)and Amand and Khosroshahr(0.0572) on UPGMA dendrogram, two population of Sarab and Heris were nested within one cluster, and Amand and Khosroshahr in other cluster. Also Bonab was completely separated from all other population.

Keywords


Sainfoin, Markers, Multiphase

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References


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